ANNEXURE II

INDICATIVE LIST OF INCIDENTS

Incidents or exposure during work in a food production plant.
Incidents or exposure during work where there is contact with animals and/or products of animal origin.
Incidents or exposure during work in health care, including isolation and post-mortem units
Incidents or exposure during work in clinical, veterinary and diagnostic laboratories.
Incidents or exposure during work in sewage purification installations.
Incidents of exposure during work in a general workplace.


ANNEXURE III

INDICATIONS CONCERNING CONTAINMENT MEASURES AND CONTAINMENT LEVELS

REGULATIONS 15(2) and 16(a) and (b)

The measures contained in this Annexure shall be applied according to the nature of the activities, the assessment of risk and the nature of the HBA concerned. (See definitions in Regulation 1.)

A.

CONTAINMENT MEASURES

 B.

CONTAINMENT LEVELS
  Level 2 Level 3 Level 4
  1. The workplace is to be separated from any other activities in the same building
 No Recommended Yes
  1. Input air and extract air to the workplace are to be filtered using High Efficiency Particulate Air Filter (HEPA) or likewise.
 No Yes, or extract air and safe discharge of air Yes, on input and extract air and safe discharge of air
  1. Access is to be restricted to authorised persons only.
 Recommended Yes Yes, via airlock
  1. The workplace is to be sealable to permit disinfection.
 No Recommended Yes
  1. Specified disinfection procedures.
 Yes Yes Yes
  1. The workplace is to be maintained at an air pressure negative to atmosphere.
 No Recommended Yes
  1. Efficient vector control, e.g. rodents and insects.
 Recommen-ded Yes Yes
  1. Surfaces impervious to water and easy to clean.
 Yes, for bench Yes, for bench and floor Yes, for bench, walls, floor and ceiling
  1. Surfaces resistant to acids, alkalis, solvents, disinfectants.
 Recommended Yes Yes
  1. Safe storage of a biological agent.
 Yes Yes Yes, secure storage
  1. An observation window, or alternative, is to be present, so that occupants can be seen.
 Recommended Recommen-ded Yes
  1. A laboratory is to contain own equipment.
 No Recommended Yes
  1. Infected material, including any animal, is to be handled in a safety cabinet or isolator or other suitable container.
 Where appropriate Yes, where infection is by airborne route Yes
  1. Incinerator for disposal of animal carcasses.
 Recommended Yes (available) Yes, on site
 


ANNEXURE IV

CONTAINMENT FOR INDUSTRIAL PROCESSES

REGULATION 16(c)

Group 1 biological agents

For work with group 1 biological agents, including life-attenuated vaccines, the principles of good occupational safety and hygiene should be observed.

Group 2, 3 and 4 agents

It may be appropriate to select and combine containment requirements from different categories below on the basis of a risk assessment related to any particular process or part of a process.

A.

CONTAINMENT MEASURES

 B.

CONTAINMENT LEVELS
  Level 2 Level 3 Level 4
  1. Viable organisms should be handled in a system which physically separates the process from the environment.
 Yes Yes Yes
  1. .Extracted air from the closed system should be treated so as to -
 minimise release prevent release prevent release
  1. Sample collection, addition of materials to a closed system and transfer of viable organisms to another closed system should be performed so as to -
 minimise release prevent release prevent release
  1. Bulk culture fluids should not be removed from the closed system unless the viable organisms have been -
 inactivated by validated means inactivated by validated chemical or physical means inactivated by validated chemical or physical means
  1. Seals should be designed as to -
 minimise release prevent release prevent release
  1. Closed systems should be located within a controlled area.
 Optional Optional Yes, and purpose-built

  1. Biohazard signs should be posted

 Optional Yes Yes

  1. Access should be restricted to nominated personnel only.

 Optional Yes Yes, via an airlock

  1. Personnel should wear protective clothing.

 Yes, work clothing Yes A complete change

  1. Decontamination and washing facilities should be provided for personnel.

 Yes Yes Yes

  1. Personnel should shower before leaving the controlled area.

 No Optional Yes

  1. Effluent from sinks and showers should be collected and inactivated before release.

 No Optional Yes

  1. The controlled area should be adequately ventilated to minimise air contamination.

 Optional Optional Yes

  1. The controlled area should be maintained at an air pressure negative to atmosphere.

 No Optional Yes

  1. Input air and extract air to the controlled area should be HEPA filtered.

 No Optional Yes

  1. The controlled area should be designed to contain spillage of the entire contents of the closed system.

 No Optional Yes

  1. The controlled area should be sealable to permit fumigation.

 No Optional Yes

  1. Effluent before final discharge.

 Inactivated by validated means Inactivated by validated chemical or physical means Inactivated by validated chemical or physical means
 

  Regulation   |    Annexure 5   |   Annesure 6